1) Denaturation of double stranded DNA involves_______________? a. It gets broken down to nucleotides b. It becomes single stranded reversibly c. It becomes single stranded irreversibly d. It becomes double stranded irreversibly
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2) Which of the following is used in recombinant DNA technology ? a. Restriction endonucleases b. PCR c. Reverse transcriptase FISH d. None of these
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3) Transcription is the synthesis of____________? a. Single stranded complimentary copy of DNA b. Double stranded complimentary copy of DNA c. Complimentary copy of RNA d. COmplimentary copy of rRNA
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4) Serum creatine kinase-3 (CK-3) is elevated in_______________? a. Muscular dystrophy b. Myocardial infarction c. Alcoholic cirrhosis d. Brain tumours
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5) Ammonia produced by brain is trapped as_____________? a. Urea b. Uric Acid c. Creatinine d. Glutamine
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6) The patient suffered from hypogondism, failure to thrive, lose of taste and unable to maintain stability. This shows the deficiency of_____________? a. Zinc b. Chromium c. Copper d. Potassium
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7) The sigma subunit of prokaryotic RNA polymerase_______________? a. Binds the antibiotic Rifampicin b. Is inhibited by a-amanitin c. Specifically recognizes the promoter site d. Is part of the core enzyme
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8) Iron absorption is decreased in presence of all except____________? a. Phytates b. Ascorbic acid c. Tannins d. Phosphates
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9) Normal serum calcium level is_______________? a. 4-6 mg/dl b. 9-11 mg/dl c. 19-21 mg/dl d. 20-30 mg/dl
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10) The normal value of serum potassium level is____________? a. 2.8-3.8 meq/L b. 3.8-5 meq/L c. 5-5.8 meq/L d. 6-7.2 meq/L
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11) Highest binding of iron in plasma is seen with___________? a. Transferrin b. Ferritin c. Hemoglobin d. Ceruloplasmin
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12) Kinase requires_______________? a. MN++ b. Cu++ c. Mg++ d. Inorganic phosphate
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13) Mutations are due to changes in_________________? a. DNA nucleotide sequence b. RNA nucleotide sequence c. Amino acid sequence of ribonuclease d. Cell walls
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14) The initiation codon for protein synthesis is_____________? a. AUG b. UAA c. UUU d. UAG
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15) Nonsense codons bring about__________________? a. Elongation of polypeptide chain b. Pre-translational modification of protein c. Initiation of protein synthesis d. Termination of protein synthesis
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16) Unwinding of DNA is done by____________? a. DNAse b. Topo isomerase c. Ligase d. Reverse transcriptase
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17) Which of the following process in involved in conversion of DNA to RNA___________? a. Conjugation b. Transduction c. Translocation d. Transcription
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18) Restriction endonuclease cleaves______________? a. Double stranded DNA b. SIngle stranded DNA c. SIngle stranded RNA d. Polypeptide
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19) Genes are_____________? a. Ribonucleic acid b. Deoxy ribonucleic acid c. Lipoproteins d. Chromo proteins
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20) DNA double helix is bound by_____________? a. Covalent bond b. Hydrogen bond c. Disulfide linkage d. Vander wall forces
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21) Which base is not found in DNA________________? a. Adenine b. Guanine c. Cytosine d. Uracil
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22) In the body, metabolism of 10 g of protein would produce approximately____________? a. 1 Kcal b. 41 Kcal c. 410 Kcal d. 4100 Kcal
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23) Alkaptonuria an inherited metabolic disorder is due to the deficiency of____________? a. Homogentisate oxidase b. Cystathionase c. Pheylalanine hydroxylase d. Tyrosine transaminase
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24) Colloidal osmotic pressure of plasma is by___________? a. Albumin b. Fibrinogen c. Globulin d. Prothrombin
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25) The daily requirement of protein for the adults is_________________? a. 6 gms b. 60 gms c. 120 gms d. 250 gms
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26) The fastest moving fraction of protein in serum When subjected to paper electrophoresis is_____________? a. Albumin b. Alpha 1 globulin c. Beta Globulin d. Gamma Globulin
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27) Most common non protein nitrogenous fraction of blood_____________? a. Urea b. Uric acid c. Urobilinogen d. Creatinin
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28) Proteins are linear polymers of amino acids, They fold into compact structures Sometimes these folded structures associate to form homo or hetero dimers Which one of the following refers to this associated form ? a. Denatured state b. Molecular aggregation c. Precipitation d. Quaternary structure
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29) The protein rich in basic amino acids, which functions in the packaging of DNA is chromosomes is______________? a. Histone b. Collagen c. Hyaluronic acid binding protein d. Fibrinogen
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30) Which of the following is not a post transcriptional modification of RNA ? a. Splicing b. 5′ capping c. 3′ polyadenylation d. Glycosylation
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